In addition to providing mutant mouse strains submitted to the MMRRC, the MU center also can provide the following to assist with your mutant mouse needs:

SERVICES OFFERED	Non-Profit/ Academic Fees	For-Profit Fees
Cryopreservation
Sperm cryopreservation	$425	$625
Embryo cryopreservation	Contact for Quote	Contact for Quote
Cryo-storage
Sperm /embryo storage (strain per year)	$306	$438
Cryo-Resuscitation
Resuscitation via  cryopreserved embryos	$3,428	$5,040
Resuscitation via cryopreserved sperm with good post-thaw motility	$4,462	$6,562
Resuscitation via cryopreserved sperm with poor post-thaw motility	$9,308	$13,688
Rederivation
Rederivation to pathogen-free status via embryo transfer	Contact for Quote
Genotyping
Genotyping (per sample)	$20	$30
Genotyping assay development	$250	$500
Genotyping assay validation	$119	$175
Microsatellite analysis (for speed congenics)	Contact for Quote
Live mice and tissues
Timed pregnant females	$1,478	$2,157
Tissue/Organ sample collection	$459	$670
Colony Management	Contact for Quote
Microbiota Characterization
16S rDNA gene sequencing	Contact for Quote

Cryopreservation

Sperm cryopreservation: submission of 3-4 males, preferably proven breeders, 10-26 weeks of age is ideal. You will receive frozen sperm aliquots in 0.3ml sterile CBS high-biosecurity straws.
Embryo cryopreservation: submission of 8-10 males, preferably proven breeders, 10-26 weeks of age, and submission of 24-32 females, preferably 7-14 weeks of age is ideal. You will receive all cryopreserved morula-stage embryos collected from the donor females you provide (~20 embryos per 0.3ml sterile CBS high-biosecurity straws).

Cryo-Resuscitation

Resuscitation via cryopreserved embryos requires submission of 40-60 cryopreserved embryos.  You will receive all recovered pups after genotyping and health monitoring procedures are performed.
Resuscitation via cryopreserved sperm that has good post-thaw progressive motility and concentration will be performed by in-vitro fertilization (IVF).  You will receive all recovered pups after genotyping and health monitoring procedures are performed.
Resuscitation via cryopreserved sperm that has poor post-thaw motility and/or low sperm concentration will be performed by intracytoplasmic sperm injection (ICSI).  You will receive all recovered pups after genotyping and health monitoring procedures are performed.

Rederivation

Rederivation to pathogen-free status via embryo transfer requires submission of 5-6 males, ideally 10-26 weeks of age and 10-12 females, ideally 7-14 weeks of age to use as oocyte-donors. You will receive all recovered pups after genotyping and health monitoring assays are performed.

Resuscitation and rederivation procedures are performed in pathogen-free barrier facility, and clean surrogate mothers purchased from commercial vendor are used for embryo transfers. You will receive the litters with confirmed genetics and pathogen-free health status.

Genotyping

Genotyping requires tail tip, ear punch or sperm aliquot.  Types of assays available include standard PCR, standard PCR followed by restriction digest or sequencing, RT-PCR, qPCR and probe-based allelic discrimination.
Genotyping assay development requires certain sequence information and a positive control sample.  Please contact us to discuss.
Validation/optimization of a genotyping assay requires an assay protocol with primer information and a positive control sample.
Microsatellite Analysis:  Low density microsatellite or SNP analysis is available for genotyping, strain identification or marker-assisted speed congenic approaches.

Live mice and tissues

Timed pregnant females are available at this price for our live colony strains only.
Tissue or organ sample collection from specific strains, preserved or fixed to your specifications. Cost includes euthanasia, necropsy and aseptic collection of one organ or tissue.  Additional samples may be collected from same animal for $10 per organ or tissue.
Colony Management accepting a breeding nucleus of mice and maintaining a colony that will produce mice for research, will include per diem and labor fees. This may also include fees for genotyping of offspring as well as a variety of other concerns, such as specialty diet, husbandry, and/or veterinary attention. Please contact us to discuss.

Microbiota Characterization

16S rDNA gene sequencing requires submission of 2 fresh fecal samples per mouse.  Samples should be frozen immediately following collection and shipped frozen.  You will receive a detailed report containing the relative abundance of all detected microbes at each taxonomic level, 2D and 3D principal component analyses, rarefaction curves, and the associated raw data.  For more information, please see here.

Fee for Service and International Orders

All Fee-for-Service and International Orders must be paid in full  beefore work can be initiated. A processing fee of $300 will be added to all international orders. 

Important notes

All MU-MMRRC services are performed with the highest scientific and biosecurity standards, and the above listed fees defray the costs associated with these services.

The procedures performed with cryopreserved materials are not 100% guaranteed.  However, MU-MMRRC has very high success rates (95-100%) if sperm or embryos were cryopreserved by us or optimally cryopreserved by the other institutions.

Fees include genotyping assays and health monitoring of surrogate mothers.  See Colony Management for health monitoring procedures. Additional cryopreservation, resuscitation or rederivation attempts are subject to extra charges and fees for these services will be determined on a case by case basis. All fees are in U.S. dollars and do not include any shipping charges.